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Cloning site: MboI (GATC); insert is underlined

 

--------T7------

TACATTATACGAAGTTATATTCGATGCGGCCGCTAATACGACTCACTATAGGGAGAAGCTTGGATC NNNNNNNNNNNNNNNNGATCCTATAGTGTCACCTAAATCGTATGCGGCCGCCCGGGCCGTCGACCA

-----Sp6-------

 

 

 

1 GATCCTATAG TGTCACCTAA ATCGTATGCG GCCGCCCGGG CCGTCGACCA

51 ATTCTCATGT TTGACAGCTT ATCATCGAAT TTCTGCCATT CATCCGCTTA

101 TTATCACTTA TTCAGGCGTA GCAACCAGGC GTTTAAGGGC ACCAATAACT

151 GCCTTAAAAA AATTACGCCC CGCCCTGCCA CTCATCGCAG TACTGTTGTA

201 ATTCATTAAG CATTCTGCCG ACATGGAAGC CATCACAAAC GGCATGATGA

251 ACCTGAATCG CCAGCGGCAT CAGCACCTTG TCGCCTTGCG TATAATATTT

301 GCCCATGGTG AAAACGGGGG CGAAGAAGTT GTCCATATTG GCCACGTTTA

351 AATCAAAACT GGTGAAACTC ACCCAGGGAT TGGCTGAGAC GAAAAACATA

401 TTCTCAATAA ACCCTTTAGG GAAATAGGCC AGGTTTTCAC CGTAACACGC

451 CACATCTTGC GAATATATGT GTAGAAACTG CCGGAAATCG TCGTGGTATT

501 CACTCCAGAG CGATGAAAAC GTTTCAGTTT GCTCATGGAA AACGGTGTAA

551 CAAGGGTGAA CACTATCCCA TATCACCAGC TCACCGTCTT TCATTGCCAT

601 ACGGAATTCC GGATGAGCAT TCATCAGGCG GGCAAGAATG TGAATAAAGG

651 CCGGATAAAA CTTGTGCTTA TTTTTCTTTA CGGTCTTTAA AAAGGCCGTA

701 ATATCCAGCT GAACGGTCTG GTTATAGGTA CATTGAGCAA CTGACTGAAA

751 TGCCTCAAAA TGTTCTTTAC GATGCCATTG GGATATATCA ACGGTGGTAT

801 ATCCAGTGAT TTTTTTCTCC ATTTTAGCTT CCTTAGCTCC TGAAAATCTC

851 GATAACTCAA AAAATACGCC CGGTAGTGAT CTTATTTCAT TATGGTGAAA

901 GTTGGAACCT CTTACGTGCC GATCAACGTC TCATTTTCGC CAAAAGTTGG

951 CCCAGGGCTT CCCGGTATCA ACAGGGACAC CAGGATTTAT TTATTCTGCG

1001 AAGTGATCTT CCGTCACAGG TATTTATTCG CGATAAGCTC ATGGAGCGGC

1051 GTAACCGTCG CACAGGAAGG ACAGAGAAAG CGCGGATCTG GGAAGTGACG

1101 GACAGAACGG TCAGGACCTG GATTGGGGAG GCGGTTGCCG CCGCTGCTGC

1151 TGACGGTGTG ACGTTCTCTG TTCCGGTCAC ACCACATACG TTCCGCCATT

1201 CCTATGCGAT GCACATGCTG TATGCCGGTA TACCGCTGAA AGTTCTGCAA

1251 AGCCTGATGG GACATAAGTC CATCAGTTCA ACGGAAGTCT ACACGAAGGT

1301 TTTTGCGCTG GATGTGGCTG CCCGGCACCG GGTGCAGTTT GCGATGCCGG

1351 AGTCTGATGC GGTTGCGATG CTGAAACAAT TATCCTGAGA ATAAATGCCT

1401 TGGCCTTTAT ATGGAAATGT GGAACTGAGT GGATATGCTG TTTTTGTCTG

1451 TTAAACAGAG AAGCTGGCTG TTATCCACTG AGAAGCGAAC GAAACAGTCG

1501 GGAAAATCTC CCATTATCGT AGAGATCCGC ATTATTAATC TCAGGAGCCT

1551 GTGTAGCGTT TATAGGAAGT AGTGTTCTGT CATGATGCCT GCAAGCGGTA

1601 ACGAAAACGA TTTGAATATG CCTTCAGGAA CAATAGAAAT CTTCGTGCGG

1651 TGTTACGTTG AAGTGGAGCG GATTATGTCA GCAATGGACA GAACAACCTA

1701 ATGAACACAG AACCATGATG TGGTCTGTCC TTTTACAGCC AGTAGTGCTC

1751 GCCGCAGTCG AGCGACAGGG CGAAGCCCTC GAGTGAGCGA GGAAGCACCA

1801 GGGAACAGCA CTTATATATT CTGCTTACAC ACGATGCCTG AAAAAACTTC

1851 CCTTGGGGTT ATCCACTTAT CCACGGGGAT ATTTTTATAA TTATTTTTTT

1901 TATAGTTTTT AGATCTTCTT TTTTAGAGCG CCTTGTAGGC CTTTATCCAT

1951 GCTGGTTCTA GAGAAGGTGT TGTGACAAAT TGCCCTTTCA GTGTGACAAA

2001 TCACCCTCAA ATGACAGTCC TGTCTGTGAC AAATTGCCCT TAACCCTGTG

2051 ACAAATTGCC CTCAGAAGAA GCTGTTTTTT CACAAAGTTA TCCCTGCTTA

2101 TTGACTCTTT TTTATTTAGT GTGACAATCT AAAAACTTGT CACACTTCAC

2151 ATGGATCTGT CATGGCGGAA ACAGCGGTTA TCAATCACAA GAAACGTAAA

2201 AATAGCCCGC GAATCGTCCA GTCAAACGAC CTCACTGAGG CGGCATATAG

2251 TCTCTCCCGG GATCAAAAAC GTATGCTGTA TCTGTTCGTT GACCAGATCA

2301 GAAAATCTGA TGGCACCCTA CAGGAACATG ACGGTATCTG CGAGATCCAT

2351 GTTGCTAAAT ATGCTGAAAT ATTCGGATTG ACCTCTGCGG AAGCCAGTAA

2401 GGATATACGG CAGGCATTGA AGAGTTTCGC GGGGAAGGAA GTGGTTTTTT

2451 ATCGCCCTGA AGAGGATGCC GGCGATGAAA AAGGCTATGA ATCTTTTCCT

2501 TGGTTTATCA AACGTGCGCA CAGTCCATCC AGAGGGCTTT ACAGTGTACA

2551 TATCAACCCA TATCTCATTC CCTTCTTTAT CGGGTTACAG AACCGGTTTA

2601 CGCAGTTTCG GCTTAGTGAA ACAAAAGAAA TCACCAATCC GTATGCCATG

2651 CGTTTATACG AATCCCTGTG TCAGTATCGT AAGCCGGATG GCTCAGGCAT

2701 CGTCTCTCTG AAAATCGACT GGATCATAGA GCGTTACCAG CTGCCTCAAA

2751 GTTACCAGCG TATGCCTGAC TTCCGCCGCC GCTTCCTGCA GGTCTGTGTT

2801 AATGAGATCA ACAGCAGAAC TCCAATGCGC CTCTCATACA TTGAGAAAAA

2851 GAAAGGCCGC CAGACGACTC ATATCGTATT TTCCTTCCGC GATATCACTT

2901 CCATGACGAC AGGATAGTCT GAGGGTTATC TGTCACAGAT TTGAGGGTGG

2951 TTCGTCACAT TTGTTCTGAC CTACTGAGGG TAATTTGTCA CAGTTTTGCT

3001 GTTTCCTTCA GCCTGCATGG ATTTTCTCAT ACTTTTTGAA CTGTAATTTT

3051 TAAGGAAGCC AAATTTGAGG GCAGTTTGTC ACAGTTGATT TCCTTCTCTT

3101 TCCCTTCGTC ATGTGACCTG ATATCGGGGG TTAGTTCGTC ATCATTGATG

3151 AGGGTTGATT ATCACAGTTT ATTACTCTGA ATTGGCTATC CGCGTGTGTA

3201 CCTCTACCTG GAGTTTTTCC CACGGTGGAT ATTTCTTCTT GCGCTGAGCG

3251 TAAGAGCTAT CTGACAGAAC AGTTCTTCTT TGCTTCCTCG CCAGTTCGCT

3301 CGCTATGCTC GGTTACACGG CTGCGGCGAG CGCTAGTGAT AATAAGTGAC

3351 TGAGGTATGT GCTCTTCTTA TCTCCTTTTG TAGTGTTGCT CTTATTTTAA

3401 ACAACTTTGC GGTTTTTTGA TGACTTTGCG ATTTTGTTGT TGCTTTGCAG

3451 TAAATTGCAA GATTTAATAA AAAAACGCAA AGCAATGATT AAAGGATGTT

3501 CAGAATGAAA CTCATGGAAA CACTTAACCA GTGCATAAAC GCTGGTCATG

3551 AAATGACGAA AGCTATCGCC ATTGCACAGT TTAATGATGA CAGCCCGGAA

3601 GCGAGGAAAA TAACCCGGCG CTGGAGAATA GGTGAAGCAG CGGATTTAGT

3651 TGGGGTTTCT TCTCAGGCTA TCAGAGATGC CGAGAAAGCA GGGCGACTAC

3701 CGCACCCGGA TATGGAAATT CGAGGACGGG TTGAGCAACG TGTTGGTTAT

3751 ACAATTGAAC AAATTAATCA TATGCGTGAT GTGTTTGGTA CGCGATTGCG

3801 ACGTGCTGAA GACGTATTTC CACCGGTGAT CGGGGTTGCT GCCCATAAAG

3851 GTGGCGTTTA CAAAACCTCA GTTTCTGTTC ATCTTGCTCA GGATCTGGCT

3901 CTGAAGGGGC TACGTGTTTT GCTCGTGGAA GGTAACGACC CCCAGGGAAC

3951 AGCCTCAATG TATCACGGAT GGGTACCAGA TCTTCATATT CATGCAGAAG

4001 ACACTCTCCT GCCTTTCTAT CTTGGGGAAA AGGACGATGT CACTTATGCA

4051 ATAAAGCCCA CTTGCTGGCC GGGGCTTGAC ATTATTCCTT CCTGTCTGGC

4101 TCTGCACCGT ATTGAAACTG AGTTAATGGG CAAATTTGAT GAAGGTAAAC

4151 TGCCCACCGA TCCACACCTG ATGCTCCGAC TGGCCATTGA AACTGTTGCT

4201 CATGACTATG ATGTCATAGT TATTGACAGC GCGCCTAACC TGGGTATCGG

4251 CACGATTAAT GTCGTATGTG CTGCTGATGT GCTGATTGTT CCCACGCCTG

4301 CTGAGTTGTT TGACTACACC TCCGCACTGC AGTTTTTCGA TATGCTTCGT

4351 GATCTGCTCA AGAACGTTGA TCTTAAAGGG TTCGAGCCTG ATGTACGTAT

4401 TTTGCTTACC AAATACAGCA ATAGTAATGG CTCTCAGTCC CCGTGGATGG

4451 AGGAGCAAAT TCGGGATGCC TGGGGAAGCA TGGTTCTAAA AAATGTTGTA

4501 CGTGAAACGG ATGAAGTTGG TAAAGGTCAG ATCCGGATGA GAACTGTTTT

4551 TGAACAGGCC ATTGATCAAC GCTCTTCAAC TGGTGCCTGG AGAAATGCTC

4601 TTTCTATTTG GGAACCTGTC TGCAATGAAA TTTTCGATCG TCTGATTAAA

4651 CCACGCTGGG AGATTAGATA ATGAAGCGTG CGCCTGTTAT TCCAAAACAT

4701 ACGCTCAATA CTCAACCGGT TGAAGATACT TCGTTATCGA CACCAGCTGC

4751 CCCGATGGTG GATTCGTTAA TTGCGCGCGT AGGAGTAATG GCTCGCGGTA

4801 ATGCCATTAC TTTGCCTGTA TGTGGTCGGG ATGTGAAGTT TACTCTTGAA

4851 GTGCTCCGGG GTGATAGTGT TGAGAAGACC TCTCGGGTAT GGTCAGGTAA

4901 TGAACGTGAC CAGGAGCTGC TTACTGAGGA CGCACTGGAT GATCTCATCC

4951 CTTCTTTTCT ACTGACTGGT CAACAGACAC CGGCGTTCGG TCGAAGAGTA

5001 TCTGGTGTCA TAGAAATTGC CGATGGGAGT CGCCGTCGTA AAGCTGCTGC

5051 ACTTACCGAA AGTGATTATC GTGTTCTGGT TGGCGAGCTG GATGATGAGC

5101 AGATGGCTGC ATTATCCAGA TTGGGTAACG ATTATCGCCC AACAAGTGCT

5151 TATGAACGTG GTCAGCGTTA TGCAAGCCGA TTGCAGAATG AATTTGCTGG

5201 AAATATTTCT GCGCTGGCTG ATGCGGAAAA TATTTCACGT AAGATTATTA

5251 CCCGCTGTAT CAACACCGCC AAATTGCCTA AATCAGTTGT TGCTCTTTTT

5301 TCTCACCCCG GTGAACTATC TGCCCGGTCA GGTGATGCAC TTCAAAAAGC

5351 CTTTACAGAT AAAGAGGAAT TACTTAAGCA GCAGGCATCT AACCTTCATG

5401 AGCAGAAAAA AGCTGGGGTG ATATTTGAAG CTGAAGAAGT TATCACTCTT

5451 TTAACTTCTG TGCTTAAAAC GTCATCTGCA TCAAGAACTA GTTTAAGCTC

5501 ACGACATCAG TTTGCTCCTG GAGCGACAGT ATTGTATAAG GGCGATAAAA

5551 TGGTGCTTAA CCTGGACAGG TCTCGTGTTC CAACTGAGTG TATAGAGAAA

5601 ATTGAGGCCA TTCTTAAGGA ACTTGAAAAG CCAGCACCCT GATGCGACCA

5651 CGTTTTAGTC TACGTTTATC TGTCTTTACT TAATGTCCTT TGTTACAGGC

5701 CAGAAAGCAT AACTGGCCTG AATATTCTCT CTGGGCCCAC TGTTCCACTT

5751 GTATCGTCGG TCTGATAATC AGACTGGGAC CACGGTCCCA CTCGTATCGT

5801 CGGTCTGATT ATTAGTCTGG GACCACGGTC CCACTCGTAT CGTCGGTCTG

5851 ATTATTAGTC TGGGACCACG GTCCCACTCG TATCGTCGGT CTGATTATCA

5901 GTCTGGAACC ACGGTCCCAC TCGTATCGTC GGTCTGATTA TTAGTCTGGG

5951 ACCACGGTCC CACTCGTATC GTCGGTCTGA TTATTAGTCT GGGACCACGA

6001 TCCCACTCGT GTTGTCGGTC TGATTATCGG TCTGGGACCA CGGTCCCACT

6051 TGTATTGTCG ATCAGACTAT CAGCGTGAGA CTACGATTCC ATCAATGCCT

6101 GTCAAGGGCA AGTATTGACA TGTCGTCGTA ACCTGTAGAA CGGAGTAACC

6151 TCGGTGTGCG GTTGTATGCC TGCTGTGGAT TGCTGCTGTG TCCTGCTTAT

6201 CCACAACATT TTGCGCACGG TTATGTGGAC AAAATACCTG GTTACCCAGG

6251 CCGTGCCGGC ACGTTAACCG GGCTGCATCC GATGCAAGTG TGTCGCTGTC

6301 GACGAGCTCG CGAGCTCGGA CATGAGGTTG CCCCGTATTC AGTGTCGCTG

6351 ATTTGTATTG TCTGAAGTTG TTTTTACGTT AAGTTGATGC AGATCAATTA

6401 ATACGATACC TGCGTCATAA TTGATTATTT GACGTGGTTT GATGGCCTCC

6451 ACGCACGTTG TGATATGTAG ATGATAATCA TTATCACTTT ACGGGTCCTT

6501 TCCGGTGATC CGACAGGTTA CGGGGCGGCG ACCTCGCGGG TTTTCGCTAT

6551 TTATGAAAAT TTTCCGGTTT AAGGCGTTTC CGTTCTTCTT CGTCATAACT

6601 TAATGTTTTT ATTTAAAATA CCCTCTGAAA AGAAAGGAAA CGACAGGTGC

6651 TGAAAGCGAG CTTTTTGGCC TCTGTCGTTT CCTTTCTCTG TTTTTGTCCG

6701 TGGAATGAAC AATGGAAGTC CGAGCTCATC GCTAATAACT TCGTATAGCA

6751 TACATTATAC GAAGTTATAT TCGATGCGGC CGCTAATACG ACTCACTATA

6801 GGGAGAAGCT TGGATC

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